AKT1 and breast carcinoma: We found that the expression of N-Ras protein was increased in MCF-7 breast cancer cells that were treated with CCL18 for 1 hr, and the phosphorylation of ERK at Thr202 and Tyr204, PI3K P85 at Tyr458 and Akt at Ser473 was concurrently induced and remained active for 7 d (Figure 2C).