ESR1 and neoplasm: Two larger studies (n = 1,322 and 168, respectively) confirmed that CD68+ macrophages were associated with higher tumor grade, estrogen receptor (ER) and progesterone receptor (PR) negativity, human epithelial growth factor receptor 2 (HER-2) positivity and a basal phenotype, but led to the conclusion that CD68 expression was not an independent prognostic factor [22, 23].