In adult C57BL/6J mice inoculated with the mouse-adapted scrapie strain 22L via the intracerebellar route [44] and sacrificed at 130 days post infection (dpi) just before the symptomatic phase, a marked increase in phosphorylated cofilin immunostaining that matched with PrPSc deposition was observed in the brain of prion-infected mice in both the cerebellar cortex (CBCX) and deep cerebellar nuclei (DCN) compared to uninfected animals (SHAM) (Fig 6A and 6B). Here, CFL1 is linked to scrapie.