We found that CSPP-L accumulated in a punctuate staining pattern at the apical region of Caco-2 cells throughout different stages of cyst formation (Fig 5) and that depletion of CSPP-L, but not Desmoplakin, is correlated with loss of ECT2 staining at apical cell-cell junctions (Fig 7). The gene discussed is DSP; the disease is cyst.