Similarly, western blotting (WB) analyses showed that the expression of HMGB1 protein was higher in BUC tissues compared with paired adjacent non-tumour tissues (0.262 ± 0.109 vs. 0.129 ± 0.111, P  < 0.05, Fig. 1B1, B2). This evidence concerns the gene HMGB1 and neoplasm.