The actin binding muscle regulatory proteins troponin and Tm that decorate actin thin filaments are also thought to be vulnerable to modification during a bout of ischemia (Gao et al., 1997; McDonough et al., 1999), therefore we examined the impact of these ischemic perturbations on myosin's ability to translocate native thin filaments (NTF) in an in vitro motility assay. The gene discussed is MYH14; the disease is ischemia.