In summary, our study showed miR-21 ASO could effectively reduce the expression of miR-21 and successively impair the proliferation and migration of human colon carcinoma cells, which was closely related to altered expression of PTEN and transduction of AKT and ERK pathway, indicating that miR-21 might be a potential target and be useful for the development of new therapeutic strategy against clinical colon carcinoma. This evidence concerns the gene AKT1 and colon carcinoma.