In the present study, we demonstrated that osthole showed anti-hepato-fibrotic effects on TAA-caused injured livers with attenuating (a) hepatic fibrosis score and collagen deposition, (b) hepatic α-SMA protein accumulation, (c) hepatic mRNA expressions of α-sma, procollagen I, icam-1, mmp2, mmp9 and mmp13, (d) hepatic translocation of p65, JunD, and Nrf-2 proteins, (e) plasma levels of ALT and AST, (f) plasma levels of several inflammation- and fibrosis-related cytokines and chemokines. Here, MMP2 is linked to hepatitis A virus infection.