In the present study, we clearly demonstrated that airway eosinophilia was significantly reduced in EC sensitized IL-25-/- mice and EC sensitized IL-33-/- mice compared with EC sensitized WT mice (Fig 3E and S7 and S8 Tables), indicating that both IL-25 and IL-33 are crucial for development of airway inflammation in the setting. This evidence concerns the gene IL25 and inflammatory response.