Figure 5e shows that knockdown of Sdhaf1 with a short hairpin (sh) RNA construct (Sdhaf1-sh) completely abrogated the RRC, with no significant effect on basal OCR at the lowest dose used of the adenoviral delivery vector (multiplicity of infection, moi 5) (Figure 5e). Increasing doses of Sdhaf1-sh, however, resulted in significant reduction (20–35%) of basal OCR that was associated with almost an equivalent degree of cell death (Figure 5f). This evidence concerns the gene SDHAF1 and infection.