Western blotting of brain cortex samples from 3 separate mice per group was done and quantified by densitometry, revealing nearly 50% reduction in aggregated Htt in the BBR-treated HD mice as compared to their untreated HD siblings (Fig 4B and 4C) (P = 0.03, T = 5.64, DF = 2). Here, HTT is linked to Huntington disease.