To further investigate the effect or role of IL-35 in the early development of MLDSTZ T1D, we examined the numbers of Tbet+ and IL-17+ cells among Foxp3+ Treg cells, Foxp3+Helios+ tTreg cells, and Foxp3+Helios− pTreg cells. Here, IKZF2 is linked to type 1 diabetes mellitus.