To validate the microarray results a qPCR assay was developed and the mRNA expression of six chosen genes (CYR61, RGS1, DUOX2, SLC6A14, EEF1A1 and DUSP1) that fluctuated reproducibly in normal and tumor colorectal tissue groups in response to ischemia were analyzed. Here, EEF1A1 is linked to neoplasm.