To further understand whether AE-SN increases the activation of cell death protein when combined with either cisplatin or doxorubicin in the two HCC cell lines, the activation of LC-3 A/B and caspase-7 was observed in HCC cells treated with 1 mg/mL of AE-SN and 5 μM cisplatin or 2 μM doxorubicin for 48 h. This evidence concerns the gene CASP7 and hepatocellular carcinoma.