Our results demonstrate, for the first time, that silencing of 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthase 1 (PAPSS1), a bi-functional enzyme that synthesizes the universal sulfate donor PAPS [11], can enhance cisplatin activity in NSCLC cell lines by inducing apoptosis and G1/S phase cell cycle arrest. Here, PAPSS1 is linked to non-small cell lung carcinoma.