Contrary to that, the synergy was not observed in HCT116 p53−/− colorectal carcinoma (Figure 2A) or in Caov3 ovary adenocarcinoma cells (Figure 2C) expressing functional TLR5 and a loss-of-function p53 mutant, demonstrating a requirement for transcriptionally active p53. This evidence concerns the gene TP53 and colorectal carcinoma.