Hence, we analyzed TGF-β protein level in the serum and mRNA expression of TGF-β and also IL-10 in the spleen during FV infection, but we could not detect any differences in the expression levels between Treg-depleted and non-depleted mice (data not shown), suggesting that TGF-β and IL-10 do not play a role in our infection model. This evidence concerns the gene TGFB1 and infection.