In contrast, cytokine secretion of all three AML patient cohorts was not impaired when stimulated with phorbol myristate acetate (PMA) and ionomycin, both for CD8+ and for CD4+ T cells, with the sole exception of decreased IFN-γ production of CD4+ T cells in AML_diag (median of 7.7 vs. 18.1; p = 0.0002; Fig. 6d). This evidence concerns the gene CD4 and acute myeloid leukemia.