Increased Cul4A protein expression was detected in the Cul4A overexpressed H28 cells compared to the EV transfected control cells 7, and mTOR protein expression was significantly increased (3.5-fold) in the mesothelioma cells when Cul4A is overexpressed (*P < 0.05, t-test; Fig. S3A and B). Here, CUL4A is linked to mesothelioma.