To analyze the potential effect of the K289R mutation on HopZ1a activity, we inoculated Arabidopsis Col-0 plants with DC3000, DC3000 expressing HopZ1a, or DC3000 expressing either the catalytically inactive HopZ1aC216A mutant or the HopZ1aK289R mutant, and compared the levels of PR1 accumulation in local tissue 48 h after infection (hpi). Here, TMEM37 is linked to infection.