When draining lymph nodes were examined (data not shown), the lymph nodes from galectin-1−/− mice were larger than those in control animals; a modest difference in lymph node size between wild type and galectin-1−/− mice was noted in sham-treated animals, but there was a dramatic increase in the size of nodes from galectin-1−/− mice compared with wild type mice in the animals with lymphedema. This evidence concerns the gene LGALS1 and lymphedema.