Figure 5B shows that the anti-tumor effects of IDG + CpG and lipoIDG + CpG were lost. In addition, the anti-tumor response was lost with IDG + CpG and lipoIDG + CpG vaccination in TC-1 tumor-bearing MyD88 knockout mice (Supplementary Fig. 1). Furthermore, we detected the Ag-specific CD8+ T cells and myeloid-derived suppressive cells (MDSCs) at the tumor site after different treatments. We found that the Ag-specific CD8+ T cell numbers were slightly higher in the IDG + CpG-treated group than in the other groups (Supplementary Fig. 2A). This evidence concerns the gene MYD88 and neoplasm.