In addition, manipulations of hippocampal ACh levels and intrahippocampal administration of ACh receptor ligands or AChE inhibitors appear to modulate anxiety (Degroot & Treit, 2002; File, Gonzalez, & Andrews, 1998; File, Kenny, & Ouagazzal, 1998), suggesting that cholinergic input to the hippocampus is important for anxiety. Here, ACHE is linked to Anxiety.