Moreover, Western blot analysis of the proteins participating in the Wnt/β-catenin pathway showed that the levels of NRIP1, β-catenin and GSK-3β phosphorylation were elevated by NOP14-overexpressing in three breast cancer cell lines (MDA-MB-231: ER-negative; MCF7 and BT474: ER-positive). The gene discussed is ESR1; the disease is breast cancer.