Examples of potential critical methylation changes include the following: GATA2 expression (methylation was positively correlated with expression among the TCGA samples) has been shown to be elevated in breast cancer and serves to repress PTEN expression [35], HOXB2 for which increased methylation is associated with bladder cancer invasiveness [36], and the long non-coding RNA HOTAIR, which demonstrated increases in methylation associated with progression and was also positively correlated with expression in TCGA tumors. This evidence concerns the gene HOXB2 and urinary bladder carcinoma.