In order to elucidate whether Syx RhoGEF activity was important for VEGF-A/NRP1-induced DJM-1 cancer cell proliferation, we constructed a lentivirus vector encoding Syx WT or a Syx mutant with a point mutation at the position of 571 Leu replaced to Glu in order to lose binding and the activation of RhoA (Marx et al., 2005). Here, VEGFA is linked to cancer.