In the second study [30] a dried methanol extract (DME) of C. genistoides, P104, was shown to bind to both estrogen receptor (ER) subtypes, transactivate an estrogen response element (ERE) containing promoter reporter construct only via ERβ, and also antagonize 17β-estradiol (E2)-induced breast cancer cell proliferation. This evidence concerns the gene ESR1 and breast cancer.