CCL2 and asthma: Subsequent qPCR results did not replicate RNA-Seq differential expression between fatal asthma- and non-asthma-derived ASM at baseline, and were only partly consistent with vitamin D effects: consistent with RNA-Seq results, CCL2 had decreased and CXCL12 had increased mRNA with vitamin D treatment for most samples, while CCL13 and IL8 manifested opposite mean effects [Fig 2].