When we categorized a cell line showing aΔCT of more than 15.0 as DCC expression-negative and a cell line with aΔCT of 15.0 or less as DCC expression-positive, only the GCIY cell line could be categorized as DCC expression-positive among the seven gastric cancer cell lines with DCC methylation; this finding was statistically non-significant. This evidence concerns the gene DCC and gastric cancer.