Transfection of CAT results in the formation of distinct hepatoblastoma and HCC-like nodules at day 90 and day 400 (figure 5D, E), while NICD transfected livers were similar in morphology to AKT/NICD-initiated livers; solely ICC at day 90 and time of morbidity (Fig 5D, E). This evidence concerns the gene AKT1 and intrahepatic cholangiocarcinoma.