Since recent human ICC transcriptome analysis revealed elevated LTβR expression in a molecularly defined proliferative subtype of patients with ICC,19 we then performed further transcriptome analysis on this cohort which revealed LTBR expression was correlated with expression of LTB (R2=0.2699) and NOTCH1 (R2=0.5081) (figure 6C). This evidence concerns the gene NOTCH1 and intrahepatic cholangiocarcinoma.