HES1 and liver cancer: Knockdown of LTβR in Huh1 and Oz cells with targeting siRNA resulted in decreased protein expression/activation of pAKTser473, CAT, NICD and Hes1 levels at 48 h post transfection (figure 6B), suggesting that LTβR signalling may be important for maintaining the activity of these oncogenes in human liver cancer cells.