In total, knockdown of 17 ciliopathy genes including AHI1, ALMS1, BBS1, BBS4, BBS7, BBS9, BBS10, BBS11 [TRIM32], BBS12, BUBR1 [BUB1B], IFT80, KIF7, NPHP1 (nephronophthisis 1), NPHP8 [RPGRIP1L], TCTN2, TMEM216 and TUB (Tubby protein homologue) resulted in delayed migration (Fig. 4a–l; Supplementary Figs 1 and 2; Supplementary Table 7). Here, TMEM216 is linked to ciliopathy.