A significant increase in the intensity of the immunostain was observed in the ‘AOM’ group for β-catenin (P = 0.01 × 10−8), iNOS (P = 0.01 × 10−5) and HSP-90 (P = 0.03 × 10−6) compared with control. The gene discussed is HSP90AA1; the disease is infectious otitis media.