We also observed thatTGFB2, TGFBR1 and SMAD2 genes (Supplementary Fig. 5i) had greater expressionin tumours with low MEG3 expression, further supporting our invitro cell culture results that MEG3 negatively regulates theTGF-β pathway genes (Fig. 3). This evidence concerns the gene TGFBR1 and neoplasm.