As shown in Figure 3A,B, the pRIG-I-EGFP dispersoid distributed throughout the cytoplasm of DF-1 and 293T cells, however, pCARDs-EGFP typically localized as dot-like structures in both the cytoplasm and nucleus, implying that pCARDs may directly interact with MAVS and recruit downstream proteins without any virus infection. This evidence concerns the gene MAVS and viral infectious disease.