In this study, we found that pCARDs induced comparative IFN synthesis with duck and goose CARDs, pigeon RIG-I showed weaker IFN-inducing activity compared with duck and goose RIG-I with or without infection, which suggests that pCARDs undergo dephosphorylation, ubiquitination, and interact with MAVS, recruit downstream signaling molecules in chicken cells. This evidence concerns the gene RIGI and infection.