However, upon IBDV TS/CJ-801 or ZB07 infection, pRIG-I-EGFP significantly increased IFN-β, Mx, and PKR mRNA expression compared with pEGFP-N1 transfection (Figure 5A–C), suggesting that pRIG-I senses influenza virus and IBDV, and triggers IFN-related genes expression in chicken cells. This evidence concerns the gene EIF2AK2 and infection.