Additionally, the significance also reached in the predictive effect of glioma histological grade; Ki-67 LI as ≥7.5 could predict high-grade WHO glioma (AUC = 0.982, 95 % CI 0.967, 0.998 with 91.76 % sensitivity and 98.5 % specificity, Fig. 4b). This evidence concerns the gene MKI67 and glioma.