Our laboratory further revealed that p66shc is a target of SIRT1 in hepatocytes and that SIRT1-mediated inhibition of p66shc attenuates oxidative stress and apoptosis during liver injury in mice and rats.17, 43 Together with our data, these findings indicate that a miR-34a/SIRT1/p66shc-dependent mechanism targeted by CA is involved in regulating oxidative stress and apoptosis in NAFLD. This evidence concerns the gene SIRT1 and metabolic dysfunction-associated steatotic liver disease.