For example, Cx43 could promote the resistance to temozolomide or cisplatin in cancer cells in a GJIC-independent manner.16, 35 Moreover, the cytoplasmic Cx32 protein itself, which failed to form GJIC, could facilitate progression of HCC.15 In this work, ‘parachute' dye-coupling assay showed no functional GJIC in HCC827 and PC9 cells with low Cx26 expression, and their GR cells with high Cx26 expression. Here, GJB2 is linked to cancer.