In the present study, we employed a multimodality functional imaging approach using three complementary imaging methods - magnetic resonance imaging (MRI), ultrasound (US) and bioluminescence imaging (BLI) to examine the potential of VDA therapy in prostate cancer alone and in combination with ADT in subcutaneous and orthotopic Myc-CaP prostate tumors. The gene discussed is MYC; the disease is prostate cancer.