Based on our angiogenesis array quantitative RT-PCR data and existing glioma angiogenesis literature [5, 23-25], we selected VEGF and FGFR as candidate mediators of the angiogenic response, observed to be significantly upregulated in the RCCS relative to monolayer cultures (Supplementary Tables 1 and 2) (KNS42: VEGFR2 +135-fold (p < 0.001), FGF +6.44-fold (p < 0.001); U87: VEGFR1 +22.63-fold (p < 0.001), FGF2 +2.85-fold (p < 0.01). Here, FLT1 is linked to central nervous system cancer.