MLH1 and medical procedure: To assess whether coordinated methylation of multiple CpG islands that are normally unmethylated plays a role in colorectal tumorigenesis, methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) was used to study eight genes firmly associated with CIMP (CACNA1G, IGF2, NEUROG1, RUNX3, SOCS1, CDKN2A, MLH1, and CRABP1).