To exclude major defects in the major innate immune pathways in the tg animals, we compared the ability of wt and C4BPxFH tg macrophages to respond to infection by culturing peritoneal macrophages with several different TLR and cGAS stimulating ligands including LPS (TLR4 ligand), Pam2CSK4 (TLR2 ligand), cytosolic dsDNA (lipofectamine + dAdT, STING ligand), Sendai virus (RIG-I ligand), live Gram-positive (GAS AP1) and Gram-negative bacteria (Neisseria gonorrhoeae; N.G.). This evidence concerns the gene STING1 and infection.