Many nonsynonymous variations were detected in the familial cardiomyopathy patients and per nonsynonymous variation in coverage of more than 30x (Table 1), including three pathogenic heterozygotic mutations (TNNI3, c.433C>G, p.Arg145Gly; LMNA, c.568C>T, p.Arg190Trp; and MYH7, c.3134G>A, p.Arg1045His). This evidence concerns the gene TNNI3 and familial cardiomyopathy.