To determine whether treatment with neurosteroids can improve mitochondrial function in AD cell culture models, ATP levels and MMP were analyzed in APP/Aβ and tau-overexpressing cells after 24 h of treatment (concentration 100 nM) with a range of steroids: progesterone (P), estradiol (E2), estrone (E1), testosterone (T), and 3α-androstanediol (3α) (Fig. 3). Here, MAPT is linked to Alzheimer disease.