To address this question, neutrophils were activated with AF or PMA for 3 h and lysed at −80 °C, and the cell extracts were used to measure G6PD activity by following NADPH production at 340 nm at 30 °C by adding NADP+ and G6P (Fig. 6, A and B). The gene discussed is G6PD; the disease is atrial fibrillation.