For example, 3-NT was found in the spinal cords of aged SOD1 G93A mice together with the presence of overnitrated proteins (actin or ATPase) in the spinal cord [82], in the motor and sensory cortices [78], and in the peripheral blood mononuclear cells [83] in transgenic mouse models of ALS and that these were observed even before the onset of the disease. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.