Treatment with 5 μm azacytidine of four RCC cell lines—769-P, Caki-1, Caki-2, and A498, was done (786-O and ACHN had low levels of methylation and were therefore excluded) and except for Caki-2, KRT19 promoter demethylation in three cell lines resulted in increased mRNA expression. This evidence concerns the gene KRT19 and renal cell carcinoma.