Apoptosis as measured with the Annexin V assay in CX+/CX− (Fig. 4c) and 4 T1 ctrl/4 T1 Hsp70 KD (Fig. 4d) tumor cells after sham (0 Gy) and 20 Gy irradiation and H1339 ctrl/H1339 HSF-1 KD and EPLC-272H ctrl/EPLC-272H HSF-1 KD cells confirmed the results of the Caspase3/7 assay. This evidence concerns the gene ANXA5 and neoplasm.