In contrast, the CRISPR/Cas9 Hsp70 knock-down mouse mammary carcinoma cell line 4 T1 did only express low levels of Hsp70 on the plasma membrane and in the cytosol, while 4 T1 ctrl and WT cells exhibited a strong membrane and cytosolic expression [17]. The gene discussed is HSPA1A; the disease is breast carcinoma.