However, mild folate deficiency significantly increased colony formation caused by oncogene expression from an average of 84 colonies per plate in the 3T3 cyclin E-expressing cells grown in a normal medium to 127 per plate in the 3T3 cyclin E-expressing cells grown under mild folate deficiency conditions (P < 0.05) (Fig5A and B). This evidence concerns the gene CCNE1 and hyperinsulinemic hypoglycemia, familial, 4.