CPA1 and infection: In our study, the levels of IgG1 increased in response to L. infantum (F/T) in all groups after infection but in the PBS control group (G3), the levels of IgG1 were significantly higher than in groups G1 and G2 immunized with L. tarentolae A2-CPA-CPB-CTE-EGFP and L. tarentolae WT, respectively.