While this protocol remains the optimal method to assess the role of hypertension candidate gene products in renal-intrinsic mechanisms as pertains to acute pressure-natriuresis relationship in mice under anesthesia, the use of continuous infusion of the hormone cocktail containing vasoactive agents may have obscured the effect of RGS2 deficiency on vascular-intrinsic mechanisms that influence renal function. The gene discussed is RGS2; the disease is hypertensive disorder.